Which of the following modifications listed below is NOT required to express a eukaryotic gene inside of a bacterium?

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Which of the following modifications listed below is NOT required to express a eukaryotic gene inside of a bacterium?

A. Concerting DNA into cDNA

B. Replacement of eukaryotic termination sequences with prokaryotic sequences

C. Making sure coding sequence is cloned downstream of prokaryotic promoter

D. Addition of poly-A tail

 
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