Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria. (1) How would you go about designing…

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Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria.

(1) How would you go about designing a mutant screen to do so?

(2) Suppose your screen identified 5 mutants that barely grew without being given Pro in the medium. How would you go about determining the number of different genes these mutants represent?

 
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