FREIHJEHC’I’ DF DEE-RESPUHWE, ItEIHEHT Lanna BEARIIG E-cELLl BDHDH BELLE BDHDH STRAIN STRAIN i “:5 ‘ i I: H-Glill] BALM: BALM: til] IIQIIH aphan QBED…

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Most dextran-specific antibodies elicited from inbred mice of the IgHa heavy chain allotype bear lambda light chains. In contrast, although mice of the IgHb allotype produce dextran-specific antibodies upon immunization, none of these antibodies bear lambda light chains. These findings led to the speculation that IgHb mice simply don’t have the heavy chain V-region genes necessary to make a dextran binding antibody with a lambda light chain. Recently, a lab used limiting dilution to compare the frequencies of lambda-bearing, dextran-responsive splenic B cells from several different bone marrow chimeras. They used irradiated BALB/c or C.B20 mice as recipients. These recipients received either sIgM+ splenocytes or B220+sIg- marrow cells from adults of either strain. [BALB/c mice have the IgHa allotype; and C.B20 mice are a congenic strain that has the IgHb allotype on the BALB/c background.] 

FREIHJEHC‘I’ DF DEE-RESPUHWE, ItEflIHEHT Lanna BEARIIG E-cELLlBDHDH BELLE BDHDH STRAIN STRAIN i “:5 ‘ i I: H-Glill]BALM: BALM: til]IIQIIH aphan QBED BALM: <1:alla BALM: REED 1TB(LBW G.B2tt <1BALM: BALM: 150Ilg- BEEIH QB!!! BALM: 1a:murmur :alII BALM: [LEE :1(LBW ELBZD :1 – why are than :haarvatlana :umpatibla wilt: previous :haawatiuna about tan-urn anti. dutran map-nun. yat in::mpatlbla wlth tha Idaa that IgH" mi:- Ia:k appraprlata ham:hain v-ganaa in make lambda baring anti-damn antlbudiaa? – Ballad :n yuur knuwladg: and thinking abnut haw th: antigan-nspnnaiv: E ::|| poulmight It: shapad, advanna a mnunahl: mndal t: explain than: data a: will an the original“rum :blanratiuna. – Design an :xpnrrirnnnt t: taat :n: aspect :f yuur mudnl.

 
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